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Prc1E and Kif4A control microtubule organization within and between large Xenopus egg asters (2018 MBoC Tim Mitchison)
conserved cytokinesis midzone proteins: Prc1 and Kif4A. Prc1E
Prc1E was required for Kif4A recruitment but not vice versa
plus-end growth slowed and terminated preferentially within interaction zones, resulting in a block to interpenetration that depended on both Prc1E and Kif4A
We propose that Prc1E and Kif4, together with catastrophe factors, promote “anti-parallel pruning” that enforces radial organization within asters and generates boundaries to microtubule growth between asters.
The plus-end–directed kinesin-family motor Kif23 (a.k.a. MKLP1) transports the furrow-stimulating factor RacGap1 (together they constitute the centralspindlin complex) to the center of the midzone, while the plus-end–directed motor Kif20A (a.k.a. MKLP2) transports the chromosome passenger complex (CPC) - Centralspindlin and the CPC then signal locally to the equatorial cortex to initiate the furrow