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Identification of XMAP215 as a microtubule-destabilizing factor in Xenopus egg extract by biochemical purification (JCB 2003 Tim Mitchison)
Microtubules (MTs) polymerized with GMPCPP, a slowly hydrolyzable GTP analogue, are stable in buffer but are rapidly depolymerized in Xenopus egg extracts. (this depolymerization is independent of three previously identified MT destabilizers (Op18, katanin, and XKCM1/KinI)
XMAP215 is identified as the novel depolymerization factor
They suggest that CPP MTs are a model for the MT pause state - an obligate intermediate between polymerization and depolymerization
XMAP215 destabilizes the pause state, acting as an antipause factor