This scientific poster explores the polyphosphate-dependent calcium signal as a marker for changing states in fibroblast cell cultures. Research conducted by investigators from Orel State University and UCL Queen Square Institute of Neurology demonstrates that inorganic polyphosphates induce a calcium response in fibroblasts, which was measured using widefield fluorescence microscopy. The intensity of this response and the proportion of responding cells vary according to the passage number and the duration of cultivation on glass, suggesting a connection to processes like cell aging and differentiation. Furthermore, the findings indicate that polyphosphate acts as a signaling molecule for metabotropic P2Y1 receptors, triggering calcium release directly from the endoplasmic reticulum through the activation of the phospholipase C enzyme.