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hybridization studies.435 Ultrastructural studies of G. vaginalis indicate that this organism has a gram-positive type❌❌ of cell wall, but the peptidoglycan layer is much thinner than that found in the cell walls of Corynebacterium, Lactobacillus, or Staphylococcus species. The peptidoglycan content of the G. vaginalis cell wall constitutes about 20% of the total cell wall weight, which is similar to that found in Enterobacteriaceae, such as Escherichia coli, where the peptidoglycan constitutes about 23% of the cell wall weight. Consequently, different strains of G. vaginalis may appear predominantly gram-positive, gram-negative, or gram-variable. Cell wall extracts of G. vaginalis do not contain compounds normally present in gram-negative cell wall lipopolysaccharides (e.g., meso-DAP, 2-keto-3-deoxy-D-manno-2-octonoic acid, hydroxy fatty acids). The absence of meso-DAP, arabinogalactans, and mycolic acids confirms that the G. vaginalis cell wall is distinct from the gram-positive cell wall type found in Corynebacterium species.Molecular studies on G. vaginalis have shown that this organism is closely related to the bifidobacteria
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